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This was corroboratedrecently by the demonstration that ATM-induced MDM2 phosphorylation at S395increased the interaction of MDM2 with p53 mRNA, leading to increased p53 trans-lation [23]

This was corroboratedrecently by the demonstration that ATM-induced MDM2 phosphorylation at S395increased the interaction of MDM2 with p53 mRNA, leading to increased p53 trans-lation [23].

Most often a strong native ligand is initiallybound to the protein and one measures how well the testligand displaces the native ligand, which is usually flu-orescently or radiolabeled.

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